R1-alcohol dehydrogenase

Intermediates

Application: Synthesis of enantiomerically pure alcohols with R-configuration

Substrate specificity: Very broad substrate specificity: aliphatic, cyclic and aromatic ketones, diketones and keto acid esters

KM VALUE [mM]: 0.99 mM Acetophenon, 0.44 mM 4-Cl-acetophenone at 30癈) and (Km 0.24 mM NADP, 0.15 mM NADPH at 30癈 and pH 7.5

pH optimum: 6.5 Reduction and 8.0 oxidation

Mr and structure: It is a tetramer of four identical subunits each of which consists of 251 amino acids and has a molecular weight of 26.6 kDa

Activity: 500 U/ml at 30癈 with acetophenone as substrate

Formulation: In glycerol

Stability: Stable at20癈