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Aflatoxins B1 ELISA test kit

  • Post Date:

    Aug 02,2017
  • Expiry Date:

    Aug 02,2018
  • Detailed Description:

    Payment Method: T/T or Western Union
    Aflatoxins B1 (AFB1) ELISA Test Kit
    Catalog No. LSY-10028

    1. Principle
    This test kit is based on the competitive enzyme immunoassay for the detection of Aflatoxins B1 in the pork and liver. The coupling antigen is pre-coated on the micro-well stripes. The Aflatoxins B1 in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Aflatoxins B1 antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Aflatoxins B1 in the sample. This value is compared to the standard curve and the Aflatoxins B1 residues is subsequently obtained.

    2. Technical specifications
    Sensitivity: 0.03 ppb
    Incubator temperature: 25℃
    Incubator time: 30min~15min
    Detection limit:
    Milk ………………………………………………………………………...0.2ppb
    Tissue……………………………………………………………………...0.8ppb
    Feed ………………………………………………………………………1.0ppb
    Urine………………………………………………………………..…….0.06ppb
    Cross-reaction rate:
    Aflatoxins B1… ……..100%
    Recovery rate:
    Milk……………………………………………………………………….… 90±15%
    Tissue…………………………………………………………………..…. 85±15%
    Feed,urine……………………………………………….……………….. 85±10%

    3. Components
    1) Micro-well strips: 12 strips with 8 removable wells each
    2) 6× standard solution (1 mL each): 0 ppb, 0.03 ppb, 0.06 ppb, 0.12 ppb, 0.24 ppb ,0.48 ppb
    3) Enzyme conjugate (7 mL) red cap
    4) Antibody working solution (7 mL) blue cap
    5) Substrate A solution (7 mL) white cap
    6) Substrate B solution (7 mL) black cap
    7) Stop solution (7 mL) yellow cap
    8) 20× concentrated washing buffer (40 mL) white cap
    9) 2× concentrated redissolving solution (50 mL) transparent cap

  • Company:

    Shenzhen Lvshiyuan Biotechnology Co.,Ltd     [ China ]        
  • Contact:

    Bella Zou
  • Tel:

    +86-755-28438788
  • Fax:

    +86-755-28938800
  • Email:

    bella.lsybt@gmail.com
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