Ammonia Assay Kit

• Post Date:

  Sep 10,2015

• Expiry Date:

  Sep 09,2016

• Detailed Description:

  Cas No. :RNF91003 Specs:95%
  Payment Method: tt
  Product Description
  
  REAGEN™ Ammonia Assay Kit is a cuvette-based enzymatic assay for the determination of Ammonia across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Ammonia directly from food samples.
  The unique features of the kit are:
  
   High sensitivity and low detection limit (0.2 ppm)
   High reproducibility
   A rapid (30 minute), robust enzyme-based assay
  
  Procedure Overview
  
  REAGEN™ Ammonia Assay Kit uses a coupled enzymatic reaction scheme to detect Ammonia in food samples. The ammonia is used as a substrate by the enzyme glutamate dehydrogenase to make glutamate. In the process, NADH is stoichiometrically converted to NAD+. This conversion, measured at 340 nm, is proportional to the level of Ammonia in the sample.
  
  The absorbance of each sample at 340 nm is measured using a spectrophotometer. The concentration of Ammonia in each sample is then directly determined from the change in NADH absorbance at 340 nm within 25 minutes.
  
  Kit Contents, Storage and Shelf Life
  
  REAGEN™ Ammonia Assay Kit has the capacity for 48 determinations or 24 determinations in duplicate. Store the kit at 4°C. The shelf life of the kit is 12 months, after receipt, when the kit is properly stored.
  
  Kit Contents Amount Storage
  Substrate Solution Mix One vial 4ºC
  Glutamate Dehydrogenase 0.25 mL 4ºC
  Ammonia Standard One vial 4ºC
  Microtiter plate 96-well plate (8 wells x 12 strips) 4ºC
  
  Sensitivity (Detection Limit)
  
  Sample Volume Detection Limit (mg/L)
  0.1 mL 4
  2 mL 0.2
  The linear range of the assay is 0.6 – 600 mg/L (ppm).
  
  Required Materials Not Provided With the Kit
  
   Spectrophotometer (340 nm)
   Low volume (0.3 mL) UV cuvettes, such as Brand Scientific catalog #7592 00
   Deionized or distilled water
   50 mL plastic conical tubes
  

• CAS Registry Number:

  RNF91003

• Company:

  REAGEN LLC     [ United States ]        

• Contact:

  Nicole

• Tel:

  8568585434

• Fax:

  1-302-269-1897

• Email:

  reagenkits@gmail.com