Ascorbic Acid Assay Kit

• Post Date:

  Sep 10,2015

• Expiry Date:

  Sep 09,2016

• Detailed Description:

  Cas No. :RNF91005 Specs:95%
  Payment Method: tt
  Product Description
  
  REAGEN™ Ascorbic Acid Assay Kit is a cuvette-based enzymatic assay for the determination of Ascorbic Acid across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Ascorbic Acid directly from food samples.
  The unique features of the kit are:
  
   High sensitivity and low detection limit (0.6 ppm)
   High reproducibility
   A rapid (25 minute), robust enzyme-based assay.
  
  Procedure Overview
  
  REAGEN™ Ascorbic Acid Assay Kit uses a specific visible, colorimetric reaction to detect ascorbic acid in food samples: ascorbic acid first reduces an electron carrier (PMS). This compound then reduces MTT to produce a colored MTT formazan product. This conversion, measured at 578 nm, is proportional to the level of ascorbic acids in the sample.
  
  The absorbance of each sample at 578 nm is measured using a spectrophotometer in a cuvette (or plate reader). The concentration of ascorbic acid in each sample is then directly determined from the change in absorbance at 578 nm within 30 minutes time.
  
  Kit Contents, Storage and Shelf Life
  
  REAGEN™ Ascorbic Acid Assay Kit has the capacity for 48 determinations or 24 determinations in duplicate. Store the kit at 4°C. The shelf life of the kit is 12 months, after receipt, when the kit is properly stored.
  
  
  Kit Contents Amount Storage
  MTT Solution 10 mL 4ºC
  PMS Reagent 2 mL 4ºC
  Ascorbate Oxidase 0.24 mL 4ºC
  Dilution Buffer 20 mL 4ºC
  
  
  
  Sensitivity (Detection Limit)
  
  Sample Volume Detection Limit (mg/L)
  0.01 mL 12
  0.2 mL 0.6
  
  The linear range of the assay is 0.6 –500 mg/L (ppm).
  
  Required Materials Not Provided With the Kit
  
   Spectrophotometer (578 nm)
   Low volume (0.3 mL) UV cuvettes, such as Brand Scientific catalog #7592 00
   Deionized or distilled water
   50 mL plastic conical tubes
  

• CAS Registry Number:

  RNF91005

• Company:

  REAGEN LLC     [ United States ]        

• Contact:

  Nicole

• Tel:

  8568585434

• Fax:

  1-302-269-1897

• Email:

  reagenkits@gmail.com