Botulinum Toxin A Strip Test 
      
      
      	
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      			Post Date:Sep 10,2015
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      			Expiry Date:Sep 09,2016
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      			Detailed Description:Cas No. :RNS 92035
			        
			        Specs:95%
 Payment Method: tt
 Product Description
 REAGEN™  Botulinum Toxin A  Strip  Test  is  a  qualitative  and  rapid  lateral  flow  assay  designed  to detect  Botulinum Toxin A  residues in meat,feed,milk,honey,milk powder,soil,vomit,feces,serum and water sample. This test is designed for field use or reference lab settings.  The unique features of the kit are:
 	 Rapid strip test method – 3 minutes
 	High sensitivity (80ppb)
 	High reproducibility
 
 Procedure Overview
 The assay uses a double antibodies sandwich  colloidal gold based format. The sample is added to the test well to allow the Botulinum Toxin A binding  protein on  the  NC  membrane   to engage  with  any  Botulinum Toxin A   present in the sample. The  gold  particles  conjugated anti-Botulinum Toxin A  will bind  to  the drug that are  bind by the Botulinum Toxin A  binding protein   at the  Test  line  (T-line),  forming a  signal  (red line)  at  that  position.  The test  is negative  if  no colored  line  appears  in  the T area.
 
 Kit Contents, Storage and Shelf Life
 The  REAGEN™  Botulinum Toxin A Strip test has  the capacity  for 48 deter-minations. The shelf life  is 24 months when the kit is properly stored.
 Kit Contents	Amount	Storage
 Reaction strip	48 strips	4°C-25°C
 Manual	1	RT
 
 
 
 
 Sample   preparation
 
 Meat/Feed/Sauce/Milk powder
 1.	Grind and mix a representative sample(according to accepted sample techniques)
 2.	Weight out 2 g of the ground sample and place into a suitable container.
 3.	 Add 5ml stroke-physiological saline solution  and shake for 10 minutes with a vortex.
 4.	Centrifuge sample for 5 minutes at 4000 rpm.
 5.	Use  the  200μL supernatant per well for the test.
 
 Honey/Milk/Serum
 1.	Take 0.1ml  sample  into  a   2ml  tube , add  0.4ml stroke-physiological saline solution and shake for 1 minutes with a vortex.
 2.	Use  the  200μL supernatant per well for the test.
 
 Soil/Vomit/Feces
 1.	Weight out 1 g of the ground sample and place into a suitable container.
 2.	 Add 5ml stroke-physiological saline solution  and shake for 10 minutes with a vortex.
 3.	Centrifuge sample for 5 minutes at 4000 rpm.
 4.	Use  the  200μL supernatant per well for the test.
 
 Water
 1.	Use  the  200μL sample  per well for the test.
 
 
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      			CAS Registry Number:RNS 92035
 
      
      	
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      			Company:REAGEN LLC
    					    [ United States ]
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      			Contact:Nicole
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      			Tel:8568585434
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      			Fax:1-302-269-1897
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      			Email:reagenkits@gmail.com
 
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