Botulinus (TypeB) PCR Diagnostic kit

• Post Date:

  Sep 10,2015

• Expiry Date:

  Sep 09,2016

• Detailed Description:

  Cas No. :RN032FP Payment Method: tt
   Product Description
  REAGEN™The botulinus(TypeB) polymerase chain reaction(PCR) diagnostic kit is designed to detect the DNA of botulinus(type B) in the feces, water and serum sample.But the result is only advisory.
  
   Procedure Overview
  REAGEN™The botulinus(TypeB) polymerase chain reaction(PCR) diagnostic kit extract DNA with a pair of botulinum (BL) B type specific primers and a specificity of fluorescent probe. After extracting DNA, and the latter in the role of Taq DNA polymerase, match with FQ - Buffer (containing magnesium 2 +, Tris - HCl, etc.), four kinds of nucleotide monomers (dNTPs), and other elements, through the PCR - fluorescent probes in vitro amplification method for botulinum type (BL) B DNA in vitro amplification, so as to achieve rapid detection of clostridium botulinum type (BL) B.
  
   Kit Contents, Storage and Shelf Life
  
  Kit Contents Amount Storage
  Nucleic acid extraction buffer 2mL -20°C
  Taq DNA Polymerase 50μL -20°C
  BL-B-qPCR MIX 1mL -20°C
  BL-B -positive control 50μL -20°C
  Negative control 250μL -20°C
  The kit’s specifications is 48 reactions/box,the validity of this kit is six months , should be preserved in - 20 ℃, avoid repeated freezing and thawing.
  
   Required Materials
  PCR instrument with double or more channels.
  
   Sample collection and storage and transportation
  
  1. Human or animal feces
  Collect fresh feces with mucus purulent blood, sealed samples, then test.
  2. Blood
  Collect fresh anticoagulant fresh blood (non heparin anticoagulation), sealed samples,then test.
  
   DNA extraction
  
  Feces
  1. Take feces sample to a centrifuge tube which contains 0.5mL saline, vortex for 1min at maximum speed.Centrifuge for 10 minutes at 13,000 x g at room temperature (20 – 25°C).
  2. Discard the supernatant and add 100μL DNA extraction buffer，vortex for 30s at maximum speed.Then heat to boil by electric furnace ,stir 10min. Centrifuge for 5 minutes at 13,000 x g at room temperature (20 – 25°C).Use the 4μL supernatant for the assay.
  
  Water
  1. Take 3mL water sample and Centrifuge for 2 minutes at 13,000 x g at room temperature (20 – 25°C).
  2. Discard the supernatant and add 100μL DNA extraction buffer，vortex for 30s at maximum speed.Then heat to boil by electric furnace ,stir 10min. Centrifuge for 5 minutes at 13,000 x g at room temperature (20 – 25°C).Use the 4μL supernatant for the assay.
  
  Serum
  1. Take 2mL fresh blood sample and allowed to separate into layers,then learn from the upper layer(plasma) and middle layer (buffy coat),Centrifuge for 2 minutes at 13,000 x g at room temperature (20 – 25°C).
  2. Discard the supernatant and add 100μL DNA extraction buffer，vortex for 30s at maximum speed.Then heat to boil by electric furnace ,stir 10min. Centrifuge for 5 minutes at 13,000 x g at room temperature (20 – 25°C).Use the 4μL supernatant for the assay.
  

• CAS Registry Number:

  RN032FP

• Company:

  REAGEN LLC     [ United States ]        

• Contact:

  Nicole

• Tel:

  8568585434

• Fax:

  1-302-269-1897

• Email:

  reagenkits@gmail.com