Dexamethasone ELISA Test Kit

• Post Date:

  Sep 10,2015

• Expiry Date:

  Sep 09,2016

• Detailed Description:

  Cas No. :RND99059 Specs:95%
  Payment Method: tt
  Product Description
  REAGEN™ Dexamethasone ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Dexamethasone, Betamethasone, Flumethasone in meat, fish, shrimp,urine and serum.
  The unique features of the kit are:
   High recovery (80-105%), rapid (less than 30 minutes), and cost-effective extraction methods.
   High sensitivity (0.1 ng/g or ppb) and low detection limit (e.g. 0.1 ng/g or ppb for meat).
   High reproducibility.
   A quick ELISA assay (less than 2 hours regardless of number of samples).
  
  Procedure Overview
  REAGEN™ Dexamethasone ELISA Test Kit is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
  
  Kit Contents, Storage and Shelf Life
  REAGEN™ Dexamethasone ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C . The shelf life is 12 months when the kit is properly stored.
  
  Kit Contents Amount Storage
  Dexamethasone-Coated Plate 1 x 96-well plate (8 wells x 12 strips) 2-8℃
  Dexamethasone Standards
  Negative control (white CAP tube)
  0.1 ng/mL (yellow CAP tube)
  0.2 ng/mL (orange CAP tube)
  0.4ng/mL (pink CAP tube)
  0.8ng/mL (purple CAP tube)
  1.6 ng/mL (blue CAP tube)
  100 ng/mL(spiking, optional, red cap tube)
  1mL
  1mL
  1mL
  1mL
  1mL
  1mL
  1mL
  
  
  2-8℃
  
  
  
  Dexamethasone Antibody #1 15mL 2-8℃
  100×HRP-Conjugated Antibody #2 2.5mL
  Antibody #2 Diluent 20mL
  20× Wash Solution 30mL
  Stop Buffer 15mL
  TMB Substrate 15mL
  Sample Balance Buffer I 10 mL
  Sample Balance Buffer Ⅱ 62g
  10×Sample Extraction Buffer 10 mL
  If you are not planning to use the kit for over 1 month ,Dexamethasone Antibody #1 and 100× HRP-Conjugated Antibody #2 at －20℃ or in a freezer is recommended.
  
  Sensitivity
  
  Sample Type Detection Limit (ng/g or ppb)
  Meat/Fish/Shrimp 0.1
  Serum 0.5
  Urine 0.5
  
  Specificity
  
  Analytes Cross-Reactivity (%)
  Dexamethasone 100
  Betamethasone 100
  Flumethasone 100
  Prednisolone 50
  Prednisone 10
  Methylprednisolone <1
  Stanozolol <0.1
  
  Required Materials Not Provided With the Kit
   Microtiter plate reader (450 nm)
   Constant temperature incubator
   Vortex mixer, (e.g. Gneie Vortex mixer from VWR)
   Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
   Pressure Blowing Concentrator
   10, 20, 100 and 1000 L pipettes
   Multi-channel pipette: 50-300 L (Optional)
  
  

• CAS Registry Number:

  RND99059

• Company:

  REAGEN LLC     [ United States ]        

• Contact:

  Nicole

• Tel:

  8568585434

• Fax:

  1-302-269-1897

• Email:

  reagenkits@gmail.com