Green Spring Chloramphenicol(CAP) ELISA Test Kit

• Post Date:

  Aug 02,2017

• Expiry Date:

  Aug 02,2018

• Detailed Description:

  Chloramphenicol(CAP) ELISA Test Kit
  1. Principle
  This test kit is based on the competitive enzyme immunoassay for the detection of Chloramphenicol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Chloramphenicol in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Chloramphenicol antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chloramphenicol in it. This value is compared to the standard curve and the Chloramphenicol concentration is subsequently obtained.
  
  2. Technical specifications
  Sensitivity: 0.05 ppb
  Detection limit:
  Intestine 0.1 ppb
  Honey 0.1 ppb
  Milk 0.05 ppb
  Urine, serum 0.1 ppb
  Tissue, liver 0.025 ppb
  Feed…………………………………………………………………………0.15 ppb
  Recovery rate
  Tissue, liver 80±10%
  Honey, intestine 70±10%
  Milk, feed 85±15%
  Urine, serum…………………………………………………………………70±10%
  Cross-reaction rate:
  Chloramphenicol 100%
  Thiamphenicol < 0.1%
  Florfeniol < 0.1%
  
  3. Components
  1） Micro-well strips: 12 strips with 8 removable wells each
  2） 6× standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb and 4.05 ppb
  3） Enzyme conjugate (12 mL) red cap
  4） Concentrated antibody working solution (1 mL) blue cap
  5） Substrate A solution (7 mL) white cap
  6） Substrate B solution (7 mL) black cap
  7） Stop solution (7 mL) yellow cap
  8） 20× concentrated washing buffer (40 mL) white cap
  9） 2× concentrated redissolving solution (50 mL) transparent cap
  

• Company:

  Shenzhen Lvshiyuan Biotechnology Co.,Ltd     [ China ]        

• Contact:

  Bella Zou

• Tel:

  +86-755-28438788

• Fax:

  +86-755-28938800

• Email:

  bella.lsybt@gmail.com