LSY-10051 Doxycycline ELISA Test Kit

• Post Date:

  Aug 02,2017

• Expiry Date:

  Aug 02,2018

• Detailed Description:

  Specs:96 wells/kit
  Price:250 USD Metric Tons
  Payment Method: T/T or Western Union
  Doxycycline ELISA Test Kit
  Catalog No. LSY-10051
  1. Principle
  This test kit is based on the competitive enzyme immunoassay for the detection of Doxycycline in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Doxycycline in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Doxycycline antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Doxycycline in it. This value is compared to the standard curve and the Doxycycline concentration is subsequently obtained.
  
  2. Technical specifications
  Sensitivity: 0.2 ppb
  Incubation Temperature: 25℃
  Incubation Time: 30min—15min
  Detection limit:
  Tissue, honey, liquid Milk(method 1) about 5ppb
  Liquid Milk(method 2) about 10ppb
  Serum about 2ppb
  Note: ppb=ng/mL or ng/g
  Cross-reaction rate:
  Doxycycline 100%
  Tetracycline 100%
  Minocycline 100%
  Chlortetracycline 180%
  Oxytetracyline 40%
  Recovery rate:
  90±15%
  
  3. Components
  1）Micro-well strips: 12 strips with 8 removable wells each
  2）10× concentrated standard solution: 0 ppb, 2ppb, 6ppb, 18ppb, 54ppb (0.5ml/bottle)
  3）11X Concentrated Enzyme conjugate (0.7 mL)
  4）Enzyme conjugate dilution (7 mL)
  5）Substrate A (7 mL)
  6）Substrate B (7 mL)
  7）Stop solution (7 mL)
  8）20× concentrated washing buffer (30 mL)
  9）20× concentrated redissolving solution (10 mL)

• Company:

  Shenzhen Lvshiyuan Biotechnology Co.,Ltd     [ China ]        

• Contact:

  Bella Zou

• Tel:

  +86-755-28438788

• Fax:

  +86-755-28938800

• Email:

  info@lsybt.com