Malachite green ELISA kit
      
      
      	
      		- 
      			Post Date:Aug 02,2017
- 
      			Expiry Date:Aug 02,2018
- 
      			Detailed Description:Payment Method: T/T or Western Union
 Malachite green ELISA kit
 Catalog No. LSY-10027
 1. Principle
 This test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Malachite green in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Malachite green antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Malachite green in it. This value is compared to the standard curve and the content of Malachite green residues is subsequently obtained.
 
 2. Technical specifications
 Sensitivity: 0.05 ppb
 Incubator temperature:  25℃
 Incubator time:  30min~30min~15min
 Detection limit
 Aquatic products e.g. Shrimp, Fish	0.5 ppb
 Water	0.1 ppb
 Recovery rate
 Shrimp, Fish, water	80%--110%
 Cross-reaction rate
 Malachite green	100%
 Leucomalachite green	0.1%
 Gentian violet 	95%
 Leucocrystal violet	0.1%
 
 3. Components
 1)	Micro-well strips: 12 strips with 8 removable wells each
 2)	6× standard solution (1 mL each, white cap): 0 ppb, 0.05 ppb, 0.15 ppb,
 0.45 ppb, 1.35 ppb and 4.05 ppb
 3)	Enzyme conjugate (12 mL)	red cap
 4)	Antibody working solution (7 mL)	blue cap
 5)	Substrate A solution (7 mL)	white cap
 6)	Substrate B solution (7 mL)……………………………………………. black cap
 7)	Stop solution (7 mL)	yellow cap
 8)	20× concentrated washing buffer (40 mL)	white cap
 9)	Oxidant (3 mL)	black cap
 10)  2× concentrated redissolving solution (50 mL)	transparent cap
 
 
      
      	
      		- 
      			Company:Shenzhen Lvshiyuan Biotechnology Co.,Ltd
    					    [ China ]
- 
      			Contact:Bella Zou
- 
      			Tel:+86-755-28438788
- 
      			Fax:+86-755-28938800
- 
      			Email:info@lsybt.com
 
      Inquiry