Nitrate/Nitrite Assay Kit

• Post Date:

  Sep 10,2015

• Expiry Date:

  Sep 09,2016

• Detailed Description:

  Cas No. :RNF91004 Specs:95%
  Payment Method: tt
  Product Description
  REAGEN™ Nitrate/Nitrite Assay Kit is a cuvette-based enzymatic assay for the determination of Nitrate/Nitrite across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Nitrate/Nitrite directly from food samples.
  The unique features of the kit are:
   High sensitivity and low detection limit (0.02 ppm)
   High reproducibility
   A rapid (45 minute), robust enzyme-based assay
  
  Procedure Overview
  REAGEN™ Nitrate/Nitrite Assay Kit uses a coupled enzymatic reaction scheme to detect nitrate and/or nitrite in food samples: nitrate in the sample is converted by nitrate reductase enzyme to make nitrite. The nitrite then reacts with sulfanilamide and N-(1-naphthyl)-ethylene-diamine to form a red diazo dye. This dye, measured at 540 nm, is proportional to the level of nitrate and/or nitrite in the sample.
  
  The absorbance of each sample at 540 nm is measured using a spectrophotometer. The concentration of nitrate /nitrite in each sample is then directly determined from the diazo dye absorbance at 540 nm within 45 minutes.
  
  Kit Contents, Storage and Shelf Life
  REAGEN™ Nitrate/Nitrite Assay Kit has the capacity for 48 determinations or 24 determinations in duplicate. Store the kit at 4°C. The shelf life of the kit is 12 months, after receipt, when the kit is properly stored.
  
  Kit Contents Amount Storage
  Buffer Solution 4 mL 4°C
  Reagent Mix 1 vial 4°C
  Nitrate Reductase 1 vial 4°C
  Color Reagent 1 3 mL 4°C
  Color Reagent 2 3 mL 4°C
  Microtiter Plate One 4°C
  
  Sensitivity (Detection Limit)
  
  Sample Volume Detection Limit (mg/L)
  0.1 mL 0.02
  The linear range of the assay is 0.02 –40 mg/L (ppm).
  
  Required Materials Not Provided With the Kit
   Spectrophotometer (578 nm)
   Low volume (0.3 mL) UV cuvettes, such as Brand Scientific catalog #7592 00
   Deionized or distilled water
   100 mL volumetric flask
   50 mL plastic conical tubes
   Carrez buffer I and Carrez buffer II (recipes included in protocol)
  
  

• CAS Registry Number:

  RNF91004

• Company:

  REAGEN LLC     [ United States ]        

• Contact:

  Nicole

• Tel:

  8568585434

• Fax:

  1-302-269-1897

• Email:

  reagenkits@gmail.com