Trichothecenes (T-2) ELISA Test Kit

• Post Date:

  May 04,2015

• Expiry Date:

  May 03,2016

• Detailed Description:

  Cas No. :BKU-10035 Quantity: 1
  Specs:Sensitivity: 0.2 ppb，96wells/kit
  Payment Method: T/T
  1. General
  T-2 toxin is a mycotoxin produced by various Fusarium. It mainly pollutes wheat, barley, corn and
  other food crops and their products, which constitute a great hazard to human health and animal
  husbandry. T2 mainly affects the function of the blood, liver, kidney, pancreatic muscle and
  lymphocyte. The clinical symptoms of T2 toxin poisoning is anorexia, vomiting, diarrhea, growth
  retardation, reproductive and neurological dysfunction etc.The use of Trichothecenes(T2) ELISA
  test kit to analyze T2 residue of samples is fast and accurate.
  This kit is a new generation of fungal toxins detection products in application of ELISA technology
  and the operation time is shorter than 60min, to minimize operating errors and work intensity.
  
  2. Principle
  This test kit is based on the competitive enzyme immunoassay for the detection of T2 in the sample. The coupling antigens are pre-coated on the micro-well stripes. The T2 in the sample and the
  coupling antigens pre-coated on the micro-well stripes compete for the anti-T2 antibodies. After the
  addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density
  (OD) value of the sample has a negative correlation with the T2 in it. This value is compared to the
  standard curve and the T2 concentration is subsequently obtained.
  
  3. Application
  This test kit can be used to detect T2 in corn, rice, wheat, beans, peanuts, oats, feed and other
  samples qualitatively and quantitatively.
  
  4. Technical specifications
  Sensitivity: 0.2 ppb
  Detection limit:
  Peanuts, corn, oats, beans, feed etc. …………………………………………………24ppb
  Wheat……………………………………………………………………………………..20ppb
  Recovery rate:
  110±15%
  Coefficient of variation: <10%
  
  5. Components
  1) Micro-well strips: 12 strips with 8 removable wells each
  2) 6 x Concentrated standards………………………………………………………1ml each
  0 ppb (zero standard), 2 ppb, 6 ppb, 18 ppb, 54 ppb, 162 ppb
  Standard working concentration: 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, 16.2ppb
  3) T2 Enzyme conjugate ……………………………………………………………… 6ml
  4) T2 Antibody working solution……………………………………………………… 6 ml
  5) Substrate A....................................................................................................... 6ml
  6) Substrate B....................................................................................................... 6ml
  7) Stop solution......................................................................................................6ml
  8) Concentrated washing buffer（10×）......................................................... ..40ml
  
  6. Materials required but not provided
  1) Equipment: microtiter plate spectrophotometer (450 nm/630nm), oscillator, vortex shaker,
  centrifuge, balance: 0.01g quantity sensitive, gaduated pipettes: 25ml, ear wash ball, funnel,
  triangle bottle with plug, polystyrene centrifuge tube: 10ml,50ml, quantitative analysis of filter paper
  2) Micropipettes: single-channel 10~100µl, 200~1000µl, multi-channel 250µl
  3) Reagents: methanol（analysis pure）, deionized water
  
  7. Sample pre-treatment
  Solution preparation before sample pre-treatment:
  1) washing buffer
  Use 1 part of concentrated washing buffer（10×） and dissolve with 9 parts of deionized water to
  obtain the ready to use washing buffer. Solution is stable for 1 month when stored at 4 °C.
  2) sample extract solution
  Use 3 part of methanol and dissolve with 2 parts of deionized water to obtain the ready to use
  sample extract.
  3) 6 T2 standards
  Take six 1.5ml centrifuge tubes, numbered from 1 to 6. Take six concentrations of standard (10 ×)
  (0ppb, 2ppb, 6ppb, 18ppb, 54ppb, 162ppb) 50μl ,add to the above six tubes, then each tube adding 450μl deionized water to dilute them by 1: 9, obtain 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, 16.2ppb the standard solution(see the following table). Configured standard solution should be used in one week (4 ℃ storage)
  
  Tube No.
  Standard working concentration (ppb)
  Standard (10X) concentration (ppb)
  Standard (10X) volume
  Deionized water volume
  1
  0
  1
  50µl
  + 450µl
  2
  0.2
  2
  50µl
  + 450µl
  3
  0.6
  6
  50µl
  + 450µl
  4
  1.8
  18
  50µl
  + 450µl
  5
  5.4
  54
  50µl
  + 450µl
  6
  16.2
  162
  50µl
  + 450µl
  
  Samples Preparation
  7.1 Preparation of Peanuts, corn, oats, beans, feed etc.
  − weigh 1g of ground sample into a suitable container and add 20 ml sample extract solution
  − shake vigorously for five minutes (manually or with shaker)
  − filter the extract through filter
  − dilute 1 part of the obtained filtrate with 5 part of distilled water or deionized water.
  − take the diluted liquid to test
  Dilution factor: 120
  7.2 Preparation of wheat sample
  − weigh 1g of ground sample into a suitable container and add 20ml of sample extract solution
  − shake vigorously for 5 minutes (manually or with shaker)
  − filter the extract through filter
  − take 1ml supernatant, add 2ml methylene dichloride, shake, centrifuge for 5min at 4000r/min,
  take methylene dichloride layer, blow to dryness at 50℃.
  − add 5 ml 10% methanol, shake evenly.
  − take the liquid to test
  Dilution factor: 100

• CAS Registry Number:

  BKU-10035

• Company:

  Shenzhen Biokau Sci-tech Co.,ltd         

• Contact:

  Samara Duan

• Tel:

  +86-0-13724287324

• Fax:

• Email:

  sales@biokau.com