Details for Lumi-Gal 530
Lumi-Gal 530
Category: Pharmaceuticals and Biochemicals
CAS NO: EC NO: Molecular Formula: Molecular Weight: Specification: Product description:
Lumi-Gal 530 is a ready-to-use formulation containing Lumigen GPD (4-methoxy-4-(3-ß-D-galactosidephenyl)spiro[1,2-dioxetane-3,2'-adamantane]) for the one-step chemiluminescent detection of ß-galactosidase. The solution contains the dioxetane, MgCl2, cetyltrimethylammonium bromide and a fluorescent enhancer in 2-amino-2-methyl-1-propanol buffer (pH 9.6). A proprietary enhancer and stabilizing agent promote the efficient operation of ß-galactosidase at this pH. This formulation is particularly useful for the chemiluminescent detection of ß-galactosidase conjugates in solution. Addition of Lumi-Gal 530 to a tube, bead or microtiter well at 37°C containing the ß-galactosidase results in chemiluminescence which increases with time and reaches a maximum after about 30 minutes. With low concentrations of enzyme, the light intensity remains constant for more than an hour. The luminescence intensity at any time point is a direct measure of the concentration of enzyme.
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