Malachite Green ELISA Test Kit
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| Post Date: | Sep 10,2015 |
| Expiry Date: | Sep 09,2016 |
| Detailed Description: |
Payment Method: REAGEN LLC REAGEN ELSIA KIT Product Description REAGEN™ Malachite green plate kit is a competitive elisa for the quantitative analysis of Malachite green in aquatic products. Procedure Overview REAGEN™Malachite green plate kit is a competitive enzyme-labeled immunoassay. Malachite green is extracted from a sample by blending or shaking with extraction solvent. The diluted Malachite green sample extract and calibrators are pipetted into the test wells followed by malachite green antibody into the test wells .Add Malachite green HRP conjugate for 30 minutes. Following wash after the incubation, a clear substrate is added to the wells and any bound enzyme conjugate causes the conversion to a blue color. After the 20 minute incubation,the reaction is stopped and the amount of color in each well is read . The color of the unknown samples is compared is compared to the color of the calibrators and the Malachite green concentration of the samples is derived. Required Materials Not Provided With the Kit 1. Laboratory quality distilled or deionized water. 2. Graduated cylinder,100ml or larger . 3. Glassware for sample extraction and extract collection. 4. Acetonitrile 5. NaCl 6. Pipet with disposable tips capable of dispensing 50μl 7. Multi-channel pipet; 8 channel capable of dispensing 50 and 100 μL 8. paper towels or equivalent absorbent material 9. Microwell plate or strip reader with 450nm filter 10. Timer. 11. Vortex mixer. |
| Company: | REAGEN LLC [ United States ] |
| Contact: | Nicole |
| Tel: | 8568585434 |
| Fax: | 1-302-269-1897 |
| Email: | reagenkits@gmail.com |
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