Malachite green ELISA kit
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| Post Date: | Aug 02,2017 |
| Expiry Date: | Aug 02,2018 |
| Detailed Description: |
Payment Method: T/T or Western Union Malachite green ELISA kit Catalog No. LSY-10027 1. Principle This test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Malachite green in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Malachite green antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Malachite green in it. This value is compared to the standard curve and the content of Malachite green residues is subsequently obtained. 2. Technical specifications Sensitivity: 0.05 ppb Incubator temperature: 25℃ Incubator time: 30min～30min～15min Detection limit Aquatic products e.g. Shrimp, Fish 0.5 ppb Water 0.1 ppb Recovery rate Shrimp, Fish, water 80%--110% Cross-reaction rate Malachite green 100% Leucomalachite green 0.1% Gentian violet 95% Leucocrystal violet 0.1% 3. Components 1） Micro-well strips: 12 strips with 8 removable wells each 2） 6× standard solution (1 mL each, white cap): 0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb and 4.05 ppb 3） Enzyme conjugate (12 mL) red cap 4） Antibody working solution (7 mL) blue cap 5） Substrate A solution (7 mL) white cap 6） Substrate B solution (7 mL)……………………………………………. black cap 7） Stop solution (7 mL) yellow cap 8） 20× concentrated washing buffer (40 mL) white cap 9） Oxidant (3 mL) black cap 10) 2× concentrated redissolving solution (50 mL) transparent cap |
| Company: | Shenzhen Lvshiyuan Biotechnology Co.,Ltd [ China ] |
| Contact: | Bella Zou |
| Tel: | +86-755-28438788 |
| Fax: | +86-755-28938800 |
| Email: | info@lsybt.com |
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