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Monensin ELISA Test Kit

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  Post Date: Sep 10,2015
  Expiry Date: Sep 09,2016
  Detailed Description: Product Description

REAGEN™ Monensin ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect monensin in feed to satisfy customer concerns about food safety. The Monensin ELISA Test Kit provides a unique extraction method, which is accurate, rapid, cost effective and easy to use. The features of the kit are:
The extraction of monensin from samples can be finished in 45 -60 minutes with a high recovery rate of 75 - 95%
A quick ELISA assay (less than 1.5 hours regardless of number of samples)
High reproducibility

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The monensin antibody has been coated in the plate wells. During the analysis, sample is added along with the monensin-horseradish peroxidase conjugate. If monensin residue is present in the sample, it will compete for the monensin antibody, thereby preventing the monensin-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the monensin residue concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN™ Monensin ELISA Test Kit has the Cyproheptadineacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Monensin Ab -coated Plate 1 x 96-well plate (8 wells x 12 strips) 2-8C
Monensin Standard Stock 300 ppb 0.8 mL 2-8C *
4x Monensin Sample Buffer 30 mL 2-8C
Monensin-HRP Conjugate 12 mL 2-8C
Monensin Clean up Mix 26 g 2-8C
20X Wash Solution ** 28 mL 2-8C
Stop Buffer ** 14 mL 2-8C
TMB Substrate ** 12 mL 2-8C


  Company: REAGEN LLC     [ United States ]        
  Contact: Nicole
  Tel: 8568585434
  Fax: 1-302-269-1897
  Email: reagenkits@gmail.com
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